Document Type

Article

Version

Published Open Access

Publication Date

8-4-2015

Publication Title

Chromatography

DOI

http://dx.doi.org/10.3390/chromatography2030502

Abstract

Large-size (4–5 µm) superficially porous particles yield lower plate heights (e.g., the minimal reduced plate height or hmin ≈ 1.5) than fully porous particles of a similar size when packed into large-bore columns. This property allows for better chromatographic performance without the higher pressures required for smaller particles. This study explores the use of such particles in microfluidic LC columns where materials and fitting pressure limits can constrain the size of particle used. The theoretically predicted performance improvements compared to fully porous particles were not demonstrated in capillary columns (with hmin ≈ 2 for both particle types), in agreement with previous studies that examined smaller superficially porous particles. Microfluidic columns were then compared to capillary columns. Capillary columns significantly outperformed microfluidic columns due to imperfections imposed by microfluidic channel asymmetry and world-to-chip connection at the optimal flow rate; however, superficially porous particles packed in microfluidic LC columns had flatter plate height versus flow rate curves indicating potential for better performance at high reduced velocities.

Comments

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Published Citation

Grinias, J. P., & Kennedy, R. T. (2015). Evaluation of 5 μm superficially porous particles for capillary and microfluidic LC columns. Chromatography, 2(3), 502-514.

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