Date of Presentation
5-5-2022 12:00 AM
College
School of Osteopathic Medicine
Poster Abstract
Overtreatment and overuse of antibiotics in healthcare and agricultural settings have contributed to the selective pressure on bacterial strains to develop resistance. Resistance can develop as a result of mutations and subsequent resistance genes that allow bacteria to survive against antibiotics. Novel silver-oxide coatings were developed and were previously demonstrated to prevent adhesion of gram-negative bacteria (Escherichia Coli and Pseudomonas Aeruginosa) to the disc, but did not prevent gram-positive bacterial adherence (Streptococcus Aureus). In order to determine whether the silver-oxide coatings are bacterial static and may be preventing progression to biofilm formation, in vivo analysis of S. Aureus attached to discs was performed. Results show that the stages of biofilm formation and infiltrating immune cells were identifiable by scanning electron microscopy (SEM). These data will be used as the foundation to compare to S. Aureus and other gram-positive bacteria attachment to coated discs.
Keywords
Pseudomonas aeruginosa, Anti-Bacterial Agents, Escherichia coli, Staphylococcus aureus, Bacteria, disilver oxide, Oxides, Biofilms
Disciplines
Bacteria | Bacterial Infections and Mycoses | Chemicals and Drugs | Medical Cell Biology | Medical Molecular Biology | Medicine and Health Sciences
Document Type
Poster
Included in
Bacteria Commons, Bacterial Infections and Mycoses Commons, Chemicals and Drugs Commons, Medical Cell Biology Commons, Medical Molecular Biology Commons
Safety and Efficacy of Silver-Coated Biomaterials in vivo
Overtreatment and overuse of antibiotics in healthcare and agricultural settings have contributed to the selective pressure on bacterial strains to develop resistance. Resistance can develop as a result of mutations and subsequent resistance genes that allow bacteria to survive against antibiotics. Novel silver-oxide coatings were developed and were previously demonstrated to prevent adhesion of gram-negative bacteria (Escherichia Coli and Pseudomonas Aeruginosa) to the disc, but did not prevent gram-positive bacterial adherence (Streptococcus Aureus). In order to determine whether the silver-oxide coatings are bacterial static and may be preventing progression to biofilm formation, in vivo analysis of S. Aureus attached to discs was performed. Results show that the stages of biofilm formation and infiltrating immune cells were identifiable by scanning electron microscopy (SEM). These data will be used as the foundation to compare to S. Aureus and other gram-positive bacteria attachment to coated discs.