Document Type
Article
Version Deposited
Published Version
Publication Date
6-1-2019
Publication Title
Regenerative Biomaterials
DOI
10.1093/rb/rbz011
Abstract
Tissue engineering of the annulus fibrosus (AF) is currently being investigated as a treatment for intervertebral disc degeneration, a condition frequently associated with low back pain. The objective of this work was to use 3D printing to generate a novel scaffold for AF repair that mimics the structural and biomechanical properties of the native tissue. Multi-layer scaffolds were fabricated by depositing polycaprolactone struts in opposing angular orientations, replicating the angle-ply arrangement of the native AF tissue. Scaffolds were printed with varied strut diameter and spacing. The constructs were characterized morphologically and by static and dynamic mechanical analyses. Scaffold surfaces were etched with unidirectional grooves and the influence on bovine AF cell metabolic activity, alignment, morphology and protein expression was studied
Recommended Citation
Christiani TR, Baroncini E, Stanzione J, & Vernengo AJ. (2019). In vitro evaluation of 3D printed polycaprolactone scaffolds with angle-ply architecture for annulus fibrosus tissue engineering. Regenerative Biomaterials, 6(3):175-184. doi:10.1093/rb/rbz011
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
