Document Type
Article
Version Deposited
Published Version
Publication Date
1-29-2016
Publication Title
Data in Brief
DOI
10.1016/j.dib.2016.01.030
Abstract
In this article we present data on the concentration of calcium as determined by Inductively Coupled Plasma (ICP) measurements. Calcium was estimated in the reagents used for native gel electrophoresis of Neuronal Calcium Sensor (NCS) proteins. NCS proteins exhibit calcium-dependent mobility shift in native gels. The sensitivity of this shift to calcium necessitated a precise determination of calcium concentrations in all reagents used. We determined the calcium concentrations in different components used along with the samples in the native gel experiments. These were: 20 mM Tris pH 7.5, loading dye and running buffer, with distilled water as reference. Calcium determinations were through ICP measurements. It was found that the running buffer contained calcium (244 nM) over the blank.
Recommended Citation
Viviano J, Wu H, Krishnan A, Ramanujachary K, Venkataraman V. Data on Final Calcium Concentration in Native Gel Reagents Determined Accurately Through Inductively Coupled Plasma Measurements. Data Brief. 2016 Jan 29;6:820-2. eCollection 2016 Mar. doi: 10.1016/j.dib.2016.01.030. PMID: 26937454. PMCID: PMC4749937.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
