Document Type

Article

Version Deposited

Published Version

Publication Date

11-28-2017

Publication Title

Journal of Electrochemical Science and Engineering

DOI

10.5599/jese.422

Abstract

Electrochemical detection of glucose was performed on carbon nanofibers containing an osmium based redox polymer and using glucose oxidase enzyme. Redox polymer assembled on the nanofibers provided a more stable support that preserved enzyme activity and promoted the electrical communication to the glassy carbon electrode. The morphologies, structures, and electrochemical behavior of the redox polymer modified nanofibers were characterized by scanning electron microscope, energy dispersive spectrometer and voltammetry. The glucose oxidase showed excellent communication with redox polymer as observed with the increased activity toward glucose. Both cyclic voltammetry and amperometry showed a linear response with glucose concentration. The linear range for glucose determination was from 1 to 12 mM with a relatively high sensitivity of 0.20±0.01 μA mM−1 for glucose oxidase in carbon nanofibers and 0.10±0.01 μA mM−1 without carbon nanofibers. The apparent Michaelis–Menten constant (Km) for glucose oxidase with carbon nanofibers was 0.99 mM. On the other hand, the Km value for the glucose oxidase without the nanofibers was 4.90 mM.

Comments

©2017 by the authors; licensee IAPC, Zagreb, Croatia. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license Attribution 4.0.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Published Citation

Mugweru, A., Mahmud, R., Ghosh, K., & Wanekaya, A. (2017). Carbon nanofiber modified with osmium based redox polymer for glucose sensing. Journal of Electrochemical Science and Engineering 7(4), 181-191.

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