M.S. Pharmaceutical Sciences
Chemistry and Biochemistry
College of Science & Mathematics
Amos Mugweru, Ph.D.
Committee Member 1
Subash Jonnalagadda, Ph.D.
Committee Member 2
Kandalam Ramanujachary Ph.D.
Analytical Chemistry, Artemisinin, Chromatography, DNA bases, LC/MS TOF, Mass spectroscopy
Biochemistry | Chemistry | Pharmacy and Pharmaceutical Sciences
Artemisinin (ART) is a sesquiterpene lactone and a popular malaria drug with potential anticancer properties. In this work, LC/TOF MS was used to investigate the reaction of ART with DNA bases and estradiol. ART-deoxyadenosine and ART-deoxycytidine interactions were studied in the presence of Fe (II) ions. ART-deoxyadenosine and ART-deoxycytidine reaction mixtures gave chromatographic signatures that remained unchanged at room temperature but grew after incubation at 37°C. The change in temperature from room temperature to 37°C was the main driver of adduct formation in these reactions. ART was found to react with Fe (II) ions as observed from several new chromatographic peaks. ART-deoxyadenosine as well as ART-deoxycytidine in the presence of Fe (II) ions resulted in the formation of new chromatographic signatures of adducts consisting of DNA bases and ART. It was clear that the addition of Fe (II) to DNA base-ART mixtures gave rise to new reaction products mediated by a different reaction mechanism. Artemisinin-estradiol reactions were studied in the presence of Fe (II) ions. Artemisinin-estradiol reaction mixtures gave chromatographic signatures that remained unchanged at room temperature but grew after incubation at 37oC. Artemisinin-estradiol in the presence of Fe (II) ions resulted in new chromatographic signatures of adducts of estradiol with artemisinin. It was clear that the addition of Fe (II) to estradiol-artemisinin mixtures gave rise to new reaction products mediated by a different mechanism. Studies of ART reactions with DNA and estradiol in vitro are key in elucidating the elusive mechanism of this drug.
Vijayan, Kogila, "ARTEMISININ AND ITS DERIVATIVES REACTIONS: CHARACTERIZATION OF THE REACTION PRODUCTS USING LC/TOF MS" (2023). Theses and Dissertations. 3114.