Understanding the Differences Between Neuronal Calcium Sensor Proteins: A Comparison of Neurocalcin Delta and Hippocalcin
PhD in Cell & Molecular Biology
Graduate School of Biomedical Sciences
School of Osteopathic Medicine
Venkateswar Venkataraman, PhD
Mikhail Anikin, PhD
Dmitry Temiakov, PhD
Neuronal Calcium-Sensor Proteins; Neurocalcin; Hippocalcin; Calcium-Binding Proteins
Cell Biology | Laboratory and Basic Science Research | Medicine and Health Sciences | Molecular and Cellular Neuroscience | Molecular Biology
Many neuronal functions, including learning and memory are driven by changes in intracellular Ca2+concentrations. The Neuronal Calcium Sensor (NCS) family of proteins is responsible for mediating the response to calcium. They are typically comprised of 4 EF hands; of which EF 2, 3, and 4 bind calcium.
Hypothesis: NCS proteins carry out unique, non-overlapping functions, and that specific characteristics of the family can be mapped to precise regions of the proteins.
Results: The effect on the following properties were investigated primarily on two highly similar NCS proteins, Neurocalcin Delta (NCALD) and Hippocalcin (HPCA): (1) Response to calcium was determined through two independent methods: change in tryptophan fluorescence and migration on a native polyacrylamide gel (CIMSA); (2) size exclusion chromatography, (3) Calcium binding, (4) Guanylate Cyclase stimulations and (5) sequence analysis. These results lead to the conclusion that specific aa locations are hyper-variable and pivotal in determining how the proteins respond.
Viviano, Jeffrey, "Understanding the Differences Between Neuronal Calcium Sensor Proteins: A Comparison of Neurocalcin Delta and Hippocalcin" (2016). Graduate School of Biomedical Sciences Theses and Dissertations. 23.
Cell Biology Commons, Laboratory and Basic Science Research Commons, Medicine and Health Sciences Commons, Molecular and Cellular Neuroscience Commons, Molecular Biology Commons
Additional committee members: Robert Nagele, PhD and Eric Moss, PhD