PhD in Cell & Molecular Biology
Graduate School of Biomedical Sciences
School of Osteopathic Medicine
Venkateswar Venkataraman, PhD
Committee Member 1
Mikhail Anikin, PhD
Committee Member 2
Dmitry Temiakov, PhD
Committee Member 3
Robert Nagele, PhD
Committee Member 4
Eric Moss, PhD
Neuronal Calcium-Sensor Proteins, Neurocalcin, Hippocalcin, Calcium-Binding Proteins
Cell Biology | Laboratory and Basic Science Research | Medicine and Health Sciences | Molecular and Cellular Neuroscience | Molecular Biology
Many neuronal functions, including learning and memory are driven by changes in intracellular Ca2+concentrations. The Neuronal Calcium Sensor (NCS) family of proteins is responsible for mediating the response to calcium. They are typically comprised of 4 EF hands; of which EF 2, 3, and 4 bind calcium.
Hypothesis: NCS proteins carry out unique, non-overlapping functions, and that specific characteristics of the family can be mapped to precise regions of the proteins.
Results: The effect on the following properties were investigated primarily on two highly similar NCS proteins, Neurocalcin Delta (NCALD) and Hippocalcin (HPCA): (1) Response to calcium was determined through two independent methods: change in tryptophan fluorescence and migration on a native polyacrylamide gel (CIMSA); (2) size exclusion chromatography, (3) Calcium binding, (4) Guanylate Cyclase stimulations and (5) sequence analysis. These results lead to the conclusion that specific aa locations are hyper-variable and pivotal in determining how the proteins respond.
Viviano, Jeffrey M., "Understanding the Differences Between Neuronal Calcium Sensor Proteins: A Comparison of Neurocalcin Delta and Hippocalcin" (2016). Graduate School of Biomedical Sciences Theses and Dissertations. 23.