Document Type

Article

Version Deposited

Not Published

Publication Date

8-2023

DOI

10.48550/arXiv.2308.08618

Abstract

Biphasic, non-sigmoidal dose-response relationships are frequently observed in biochemistry and pharmacology, but they are not always analyzed with appropriate statistical methods. Here, we examine curve fitting methods for “hormetic” dose-response relationships where low and high doses of an effector produce opposite responses. We provide the full dataset used for modeling, and we provide the code for analyzing the dataset in SAS using two established mathematical models of hormesis, the Brain-Cousens model and the Cedergreen model. We show how to obtain and interpret curve parameters such as the ED50 that arise from modeling, and we discuss how curve parameters might change in a predictable manner when the conditions of the dose-response assay are altered. In addition to modeling the raw dataset that we provide, we also model the dataset after applying common normalization techniques, and we indicate how this affects the parameters that are associated with the fit curves. The Brain-Cousens and Cedergreen models that we used for curve fitting were similarly effective at capturing quantitative information about the biphasic dose-response relationships.

Comments

Also available at https://arxiv.org/abs/2308.08618

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Published Citation

Abbaraju VD, Robinson TL, Weiser BP. Modeling biphasic, non-sigmoidal dose-response relationships: comparison of Brain-Cousens and Cedergreen models for a biochemical dataset. arXiv (q-bio.QM). August 16, 2023. https://doi.org/10.48550/arXiv.2308.08618

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