Date of Presentation
5-6-2021 12:00 AM
College
School of Osteopathic Medicine
Poster Abstract
Pseudomonas aeruginosa (P aeruginosa) is an opportunistic bacterium commonly found in wound infections and airways of cystic fibrosis patients P aeruginosa readily forms biofilms which can reduce the efficacy of antibiotics used to eradicate the pathogen We have previously shown that a Specialized Pro resolving Mediator ( Lipoxin A 4 (LxA 4 is a quorum sensing inhibitor which can reduce P aeruginosa virulence In this study, we examined the direct actions of LxA 4 and RvD 2 on P aeruginosa biofilm formation and virulence gene expression The influence of LxA 4 on antibiotic efficacy and the combined effects on biofilm formation were also investigated LxA 4 and RvD 2 reduced P aeruginosa biofilm formation and virulence gene expression LxA 4 increased ciprofloxacin inhibition on biofilm formation but did not affect ciprofloxacin’s action on non adherent bacteria On the other hand, LxA 4 increased bacterial killing action of imipenem but did not affect imipenem’s action on biofilm We also found that LxA 4 can increase ciprofloxacin’s bacterial killing ability in established biofilm Together these results suggest that LxA 4 has direct effects on P aeruginosa biofilm formation and can increase antibiotic efficacy directly.
Keywords
Pseudomonas aeruginosa, lipoxins, Lipoxin A 4, biofilms, Anti-Bacterial Agents, gene expression
Disciplines
Bacterial Infections and Mycoses | Bacteriology | Biological Phenomena, Cell Phenomena, and Immunity | Medical Cell Biology | Medicinal and Pharmaceutical Chemistry | Medicine and Health Sciences
Document Type
Poster
Included in
Bacterial Infections and Mycoses Commons, Bacteriology Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Medical Cell Biology Commons, Medicinal and Pharmaceutical Chemistry Commons
Lipoxin A 4 (LxA 4 ) Promotes Reduction and Antibiotic Efficacy Against Pseudomonas aeruginosa Biofilm
Pseudomonas aeruginosa (P aeruginosa) is an opportunistic bacterium commonly found in wound infections and airways of cystic fibrosis patients P aeruginosa readily forms biofilms which can reduce the efficacy of antibiotics used to eradicate the pathogen We have previously shown that a Specialized Pro resolving Mediator ( Lipoxin A 4 (LxA 4 is a quorum sensing inhibitor which can reduce P aeruginosa virulence In this study, we examined the direct actions of LxA 4 and RvD 2 on P aeruginosa biofilm formation and virulence gene expression The influence of LxA 4 on antibiotic efficacy and the combined effects on biofilm formation were also investigated LxA 4 and RvD 2 reduced P aeruginosa biofilm formation and virulence gene expression LxA 4 increased ciprofloxacin inhibition on biofilm formation but did not affect ciprofloxacin’s action on non adherent bacteria On the other hand, LxA 4 increased bacterial killing action of imipenem but did not affect imipenem’s action on biofilm We also found that LxA 4 can increase ciprofloxacin’s bacterial killing ability in established biofilm Together these results suggest that LxA 4 has direct effects on P aeruginosa biofilm formation and can increase antibiotic efficacy directly.