College
School of Osteopathic Medicine
Keywords
Pseudomonas aeruginosa, lipoxins, Lipoxin A 4, biofilms, Anti-Bacterial Agents, gene expression
Date of Presentation
5-6-2021 12:00 AM
Poster Abstract
Pseudomonas aeruginosa (P aeruginosa) is an opportunistic bacterium commonly found in wound infections and airways of cystic fibrosis patients P aeruginosa readily forms biofilms which can reduce the efficacy of antibiotics used to eradicate the pathogen We have previously shown that a Specialized Pro resolving Mediator ( Lipoxin A 4 (LxA 4 is a quorum sensing inhibitor which can reduce P aeruginosa virulence In this study, we examined the direct actions of LxA 4 and RvD 2 on P aeruginosa biofilm formation and virulence gene expression The influence of LxA 4 on antibiotic efficacy and the combined effects on biofilm formation were also investigated LxA 4 and RvD 2 reduced P aeruginosa biofilm formation and virulence gene expression LxA 4 increased ciprofloxacin inhibition on biofilm formation but did not affect ciprofloxacin’s action on non adherent bacteria On the other hand, LxA 4 increased bacterial killing action of imipenem but did not affect imipenem’s action on biofilm We also found that LxA 4 can increase ciprofloxacin’s bacterial killing ability in established biofilm Together these results suggest that LxA 4 has direct effects on P aeruginosa biofilm formation and can increase antibiotic efficacy directly.
Disciplines
Bacterial Infections and Mycoses | Bacteriology | Biological Phenomena, Cell Phenomena, and Immunity | Medical Cell Biology | Medicinal and Pharmaceutical Chemistry | Medicine and Health Sciences
Included in
Bacterial Infections and Mycoses Commons, Bacteriology Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Medical Cell Biology Commons, Medicinal and Pharmaceutical Chemistry Commons
Lipoxin A 4 (LxA 4 ) Promotes Reduction and Antibiotic Efficacy Against Pseudomonas aeruginosa Biofilm
Pseudomonas aeruginosa (P aeruginosa) is an opportunistic bacterium commonly found in wound infections and airways of cystic fibrosis patients P aeruginosa readily forms biofilms which can reduce the efficacy of antibiotics used to eradicate the pathogen We have previously shown that a Specialized Pro resolving Mediator ( Lipoxin A 4 (LxA 4 is a quorum sensing inhibitor which can reduce P aeruginosa virulence In this study, we examined the direct actions of LxA 4 and RvD 2 on P aeruginosa biofilm formation and virulence gene expression The influence of LxA 4 on antibiotic efficacy and the combined effects on biofilm formation were also investigated LxA 4 and RvD 2 reduced P aeruginosa biofilm formation and virulence gene expression LxA 4 increased ciprofloxacin inhibition on biofilm formation but did not affect ciprofloxacin’s action on non adherent bacteria On the other hand, LxA 4 increased bacterial killing action of imipenem but did not affect imipenem’s action on biofilm We also found that LxA 4 can increase ciprofloxacin’s bacterial killing ability in established biofilm Together these results suggest that LxA 4 has direct effects on P aeruginosa biofilm formation and can increase antibiotic efficacy directly.
