Date of Presentation
5-6-2021 12:00 AM
College
School of Osteopathic Medicine
Poster Abstract
It has been demonstrated that silver oxide coatings designed by our collaborators are able to prevent E. coli and P. aeruginosa attachment to biomaterials in vivo. These findings demonstrate that such coatings show promise in preventing the development of biofilm on biodevices. However, it is unknown if the use of silver oxide in this fashion is toxic in vivo. The goal of this project was to determine whether our silver oxide coatings are safe to use in vivo. To assess the toxicity of our silver oxide formula, mice were implanted with either silver oxide coated titanium discs or uncoated titanium discs. Blood samples were drawn at pre-determined time points in order to determine AST and ALT levels via ELISA assay. Preliminary results demonstrate no acute liver injury after 3 months with the discs. However, it appears as if silver is accumulating in tissues over time. Histological analysis at one year shows evidence of simple steatosis in livers. While our group is continuing to investigate the safety and efficacy of these silver oxides coatings in vivo, preliminary data shows that they may have some toxicity and the silver oxide formula may need to be altered and retested.
Keywords
biofilms, silver oxide, toxicity, biodevices
Disciplines
Animal Experimentation and Research | Biochemistry | Biomedical and Dental Materials | Biotechnology | Laboratory and Basic Science Research | Medicine and Health Sciences | Molecular Biology
Document Type
Poster
Included in
Animal Experimentation and Research Commons, Biochemistry Commons, Biomedical and Dental Materials Commons, Biotechnology Commons, Laboratory and Basic Science Research Commons, Molecular Biology Commons
Safety of Silver Oxide Coated Biomaterials in Mice
It has been demonstrated that silver oxide coatings designed by our collaborators are able to prevent E. coli and P. aeruginosa attachment to biomaterials in vivo. These findings demonstrate that such coatings show promise in preventing the development of biofilm on biodevices. However, it is unknown if the use of silver oxide in this fashion is toxic in vivo. The goal of this project was to determine whether our silver oxide coatings are safe to use in vivo. To assess the toxicity of our silver oxide formula, mice were implanted with either silver oxide coated titanium discs or uncoated titanium discs. Blood samples were drawn at pre-determined time points in order to determine AST and ALT levels via ELISA assay. Preliminary results demonstrate no acute liver injury after 3 months with the discs. However, it appears as if silver is accumulating in tissues over time. Histological analysis at one year shows evidence of simple steatosis in livers. While our group is continuing to investigate the safety and efficacy of these silver oxides coatings in vivo, preliminary data shows that they may have some toxicity and the silver oxide formula may need to be altered and retested.