Date of Presentation
5-6-2021 12:00 AM
College
School of Osteopathic Medicine
Poster Abstract
Replication Protein A (RPA) is a single stranded DNA binding protein which stabilizes ssDNA for replication and repair. One function of RPA is to bind the DNA repair enzyme uracil DNA glycosylase (UNG2) and direct its activity towards ssDNA dsDNA junctions.
UNG2 removes uracil bases from DNA which can appear through dUMP misincorporation or through cytosine deamination. If uracil is present instead of a cytosine, then the original GC pair becomes a GU pair. The uracil will then base pair to adenine in the replicated daughter strand. This results in a GC → AT mutation that could contribute to cancer formation.
RPA is known to target UNG2 towards individual uracil bases. We hypothesize that RPA will target UNG2 to uracil bases in DNA regardless of the number of uracils in the DNA strand.
Keywords
Uracil, Replication Protein A, DNA, Mutation
Disciplines
Genetic Processes | Medical Molecular Biology | Medicine and Health Sciences | Molecular Biology | Neoplasms
Document Type
Poster
Included in
Genetic Processes Commons, Medical Molecular Biology Commons, Molecular Biology Commons, Neoplasms Commons
Replication Protein A (RPA) Targeting of Uracil DNA Glycosylase (UNG2)
Replication Protein A (RPA) is a single stranded DNA binding protein which stabilizes ssDNA for replication and repair. One function of RPA is to bind the DNA repair enzyme uracil DNA glycosylase (UNG2) and direct its activity towards ssDNA dsDNA junctions.
UNG2 removes uracil bases from DNA which can appear through dUMP misincorporation or through cytosine deamination. If uracil is present instead of a cytosine, then the original GC pair becomes a GU pair. The uracil will then base pair to adenine in the replicated daughter strand. This results in a GC → AT mutation that could contribute to cancer formation.
RPA is known to target UNG2 towards individual uracil bases. We hypothesize that RPA will target UNG2 to uracil bases in DNA regardless of the number of uracils in the DNA strand.