Date of Presentation

5-5-2022 12:00 AM

College

School of Osteopathic Medicine

Poster Abstract

Objective: Previous work has shown that alternate site (RV apex) results in myocardial dysfunction. With the development of tools to place endocardial pacing leads in locations that create physiological pacing activation, we sought to evaluate how ventricular trans-septal or left ventricular apical placement pacing differs from right atrial pacing. We will evaluate how these chronic pacing modes impact the PR and RF at baseline, 0, and 16 weeks in the canine heart.

Methods: Quantitative analysis will be performed on previously generated data. The data set includes pacing of 15 dogs total (8 with trans-septal leads & 7 with left ventricular apical leads). GraphPad Prism software will be used for the statistical analysis of the data. The recirculation fraction value will be calculated as the slope of dP/dtmax following the extra-systole. The potentiation ratio will be calculated by normalizing dP/dtmax from the first beat following extrasystole to the steady state dP/dtmax. From these values we will uncover any changes in the force interval relationship which can be attributed to differences in calcium handling associated with different pacing sites.

Results/Conclusions: The results of this study are expected to demonstrate how alternate site pacing impacts intracellular calcium handling using the post-extrasystolic cardiac cycles. We expect some loss in the force interval relationship following the 16 weeks of alternant site pacing due to the alterations in Ca+2 handling that accompanies the post-extrasystolic beat. Through this project we hope to gain a deeper understanding of cardiac function while simultaneously hoping to provide evidence for the reintroduction of the RF and PR parameters into the clinical space.

Keywords

Cardiac Electrophysiology, Cardiology, Cell Physiological Phenomena

Disciplines

Cardiovascular Diseases | Circulatory and Respiratory Physiology | Disease Modeling | Equipment and Supplies | Laboratory and Basic Science Research | Medical Biochemistry | Medicine and Health Sciences | Physiological Processes

Document Type

Poster

COinS
 
May 5th, 12:00 AM

Alternate Site Pacing and the Impact on Intracellular Calcium Handling During the Post-Extrasystolic Cardiac Cycle

Objective: Previous work has shown that alternate site (RV apex) results in myocardial dysfunction. With the development of tools to place endocardial pacing leads in locations that create physiological pacing activation, we sought to evaluate how ventricular trans-septal or left ventricular apical placement pacing differs from right atrial pacing. We will evaluate how these chronic pacing modes impact the PR and RF at baseline, 0, and 16 weeks in the canine heart.

Methods: Quantitative analysis will be performed on previously generated data. The data set includes pacing of 15 dogs total (8 with trans-septal leads & 7 with left ventricular apical leads). GraphPad Prism software will be used for the statistical analysis of the data. The recirculation fraction value will be calculated as the slope of dP/dtmax following the extra-systole. The potentiation ratio will be calculated by normalizing dP/dtmax from the first beat following extrasystole to the steady state dP/dtmax. From these values we will uncover any changes in the force interval relationship which can be attributed to differences in calcium handling associated with different pacing sites.

Results/Conclusions: The results of this study are expected to demonstrate how alternate site pacing impacts intracellular calcium handling using the post-extrasystolic cardiac cycles. We expect some loss in the force interval relationship following the 16 weeks of alternant site pacing due to the alterations in Ca+2 handling that accompanies the post-extrasystolic beat. Through this project we hope to gain a deeper understanding of cardiac function while simultaneously hoping to provide evidence for the reintroduction of the RF and PR parameters into the clinical space.

 

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