College
Rowan-Virtua School of Osteopathic Medicine
Keywords
nanomedicine, nanoparticles, cerium oxide nanoparticles, sustainable biomaterials, bone differentiation, enzyme-mimetics
Date of Presentation
5-1-2025 12:00 AM
Poster Abstract
Cerium oxide nanoparticles (CNPs) are recognized for their ability to scavenge reactive oxygen species (ROS), making them promising candidates for mitigating oxidative damage in biomedical applications. However, many conventional synthesis methods limit the formation of Ce³⁺ states, which are critical for this antioxidant function. Building upon previous findings that glucose can promote Ce³⁺ formation, this study investigates the broader impact of both reducing (glucose, fructose, galactose) and non-reducing (dextran, sucrose, cyclodextrin) natural sugars on CNP synthesis. CNPs were synthesized in the presence of these sugars, and the surface densities of Ce³⁺ were measured. Results showed that reducing sugars significantly increased Ce³⁺ surface density compared to controls, enhancing the redox-active nature of the nanoparticles. Superoxide dismutase (SOD) assays further demonstrated a correlation between higher Ce³⁺ ratios and increased biological activity, affirming the functional importance of redox modulation. Biological evaluation in human bone marrow-derived mesenchymal stem cells (hMSCs) revealed no cytotoxic effects from any CNP formulation. Notably, sugar-modified CNPs at low concentrations enhanced alkaline phosphatase (ALP) activity and calcium deposition, as confirmed by ALP and Alizarin Red S (ARS) assays, key indicators of osteogenic differentiation. Overall, the study underscores the therapeutic potential of Ce³⁺-rich, sugar-modified CNPs for promoting bone growth and broadens their applicability in regenerative medicine.
Disciplines
Biochemistry, Biophysics, and Structural Biology | Biotechnology | Laboratory and Basic Science Research | Medicinal Chemistry and Pharmaceutics | Medicine and Health Sciences
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Biotechnology Commons, Laboratory and Basic Science Research Commons, Medicinal Chemistry and Pharmaceutics Commons, Medicine and Health Sciences Commons
Direct Synthesis of Cerium Oxide Nanoparticles in Reducing Sugars: Enhanced Ce3+ Surface Density for Biological Application
Cerium oxide nanoparticles (CNPs) are recognized for their ability to scavenge reactive oxygen species (ROS), making them promising candidates for mitigating oxidative damage in biomedical applications. However, many conventional synthesis methods limit the formation of Ce³⁺ states, which are critical for this antioxidant function. Building upon previous findings that glucose can promote Ce³⁺ formation, this study investigates the broader impact of both reducing (glucose, fructose, galactose) and non-reducing (dextran, sucrose, cyclodextrin) natural sugars on CNP synthesis. CNPs were synthesized in the presence of these sugars, and the surface densities of Ce³⁺ were measured. Results showed that reducing sugars significantly increased Ce³⁺ surface density compared to controls, enhancing the redox-active nature of the nanoparticles. Superoxide dismutase (SOD) assays further demonstrated a correlation between higher Ce³⁺ ratios and increased biological activity, affirming the functional importance of redox modulation. Biological evaluation in human bone marrow-derived mesenchymal stem cells (hMSCs) revealed no cytotoxic effects from any CNP formulation. Notably, sugar-modified CNPs at low concentrations enhanced alkaline phosphatase (ALP) activity and calcium deposition, as confirmed by ALP and Alizarin Red S (ARS) assays, key indicators of osteogenic differentiation. Overall, the study underscores the therapeutic potential of Ce³⁺-rich, sugar-modified CNPs for promoting bone growth and broadens their applicability in regenerative medicine.
