Date of Presentation

4-23-2024 9:00 AM

College

College of Science & Mathematics

Faculty Sponsor(s)

Dr. Lark Perez

Poster Abstract

• Vibrio cholerae is a gram-negative anaerobic bacteria that inhabits brackish or saltwater areas.

• Causative agent of cholera, which results in acute diarrhea and dehydration.

• Uses quorum sensing, a cell density dependent method of communicating with other bacteria and regulating its entire lifecycle of infection. (gene expression of functions such as biofilm formation, virulence, and more)

• Vibrio cholerae determines bacterial population based on the secretion of several signaling molecules called an autoinducer from others of their kind.

• Upon adequate accumulation, they can deactivate their virulence and begin to leave their host’s body to continue infecting other organisms.

•The goal of this experiment is to identify the structure of and isolate one of the autoinducer molecules. Current studies suggest there are four chemical inputs in V. cholerae, two are known (Ea-CAI1/CAI-1 circuit and the AI-2 circuit).

Student Keywords

quorum sensing, v. cholerae

Disciplines

Chemistry

Document Type

Poster

Included in

Chemistry Commons

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Apr 23rd, 9:00 AM

Identifying V. Cholerae's Autoinducer to Manipulate Its Quorum Sensing

• Vibrio cholerae is a gram-negative anaerobic bacteria that inhabits brackish or saltwater areas.

• Causative agent of cholera, which results in acute diarrhea and dehydration.

• Uses quorum sensing, a cell density dependent method of communicating with other bacteria and regulating its entire lifecycle of infection. (gene expression of functions such as biofilm formation, virulence, and more)

• Vibrio cholerae determines bacterial population based on the secretion of several signaling molecules called an autoinducer from others of their kind.

• Upon adequate accumulation, they can deactivate their virulence and begin to leave their host’s body to continue infecting other organisms.

•The goal of this experiment is to identify the structure of and isolate one of the autoinducer molecules. Current studies suggest there are four chemical inputs in V. cholerae, two are known (Ea-CAI1/CAI-1 circuit and the AI-2 circuit).