Document Type
Article
Version Deposited
Published Version
Publication Date
3-12-2021
Publication Title
Biomolecules
DOI
10.3390/biom11030421
Abstract
Antimicrobial peptides (AMPs) present a promising scaffold for the development of potent antimicrobial agents. Substitution of tryptophan by non-natural amino acid Azulenyl-Alanine (AzAla) would allow studying the mechanism of action of AMPs by using unique properties of this amino acid, such as ability to be excited separately from tryptophan in a multi-Trp AMPs and environmental insensitivity. In this work, we investigate the effect of Trp→AzAla substitution in antimicrobial peptide buCATHL4B (contains three Trp side chains). We found that antimicrobial and bactericidal activity of the original peptide was preserved, while cytocompatibility with human cells and proteolytic stability was improved. We envision that AzAla will find applications as a tool for studies of the mechanism of action of AMPs. In addition, incorporation of this non-natural amino acid into AMP sequences could enhance their application properties.
Recommended Citation
D’Souza, Areetha R., Matthew R. Necelis, Alona Kulesha, Gregory A. Caputo, and Olga V. Makhlynets 2021. "Beneficial Impacts of Incorporating the Non-Natural Amino Acid Azulenyl-Alanine into the Trp-Rich Antimicrobial Peptide buCATHL4B" Biomolecules 11, no. 3: 421. https://doi.org/10.3390/biom1103042
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Comments
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.