Document Type
Article
Version Deposited
Published Version
Publication Date
11-2024
Publication Title
Journal of Chromatography Open
DOI
10.1016/j.jcoa.2024.100190
Abstract
HPLC remains one of the most widely used measurement techniques for chemical analysis. Capillary LC, which utilizes narrow diameter columns operated at lower flow rates than analytical-scale LC, continues to gain adoption based on its reduced mobile phase consumption and increased sensitivity when coupled to MS detection. This tutorial offers practical insights into the most critical aspects of translating analytical-scale separations to the capillary scale. The selection of pumping systems, detectors, and the potential for performance loss due to extra-column effects are examined within the context of separations using columns with inner diameters ≤ 0.3 mm. Column choices within this diameter range are also detailed, both in terms of stationary phase support options and general commercial availability. The impact of these various factors on the effective development/translation of LC methods down to flow rates under 10 µL/min is described to provide readers with a basis for implementing these strategies within their own analytical workflows.
Recommended Citation
Foster, Samuel W.; Piccolo, Christopher; Hanson, Eliza K.; and Grinias, James P., "Considerations for method development and method translation in capillary liquid chromatography: A tutorial" (2024). College of Science & Mathematics Departmental Research. 358.
https://rdw.rowan.edu/csm_facpub/358
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Comments
Copyright 2024 The Authors Published by Elsevier.