Document Type
Article
Version Deposited
Published Version
Publication Date
8-31-2023
Publication Title
Frontiers in Bioengineering and Biotechnology
DOI
10.3389/fbioe.2023.1193970
Abstract
Light-assisted bioprinted gelatin methacryloyl (GelMA) constructs have been used for cell-laden microtissues and organoids. GelMA can be loaded by desired cells, which can regulate the biophysical properties of bioprinted constructs. We study how the degree of methacrylation (MA degree), GelMA mass concentration, and cell density change mass transport properties. We introduce a fluorescent-microscopy-based method of biotransport testing with improved sensitivity compared to the traditional particle tracking methods. The diffusion capacity of GelMA with a higher MA significantly decreased compared to a lower MA. Opposed to a steady range of linear elastic moduli, the diffusion coefficient in GelMA varied when cell densities ranged from 0 to 10 × 106 cells/ml. A comparative study of different cell sizes showed a higher diffusivity coefficient for the case of larger cells. The results of this study can help bioengineers and scientists to better control the biotransport characteristics in light-assisted bioprinted microtissues and organoids.
Recommended Citation
Dogan E, Holshue C, Bhusal A, Shukla R and Miri AK (2023) Cell encapsulation in gelatin methacryloyl bioinks impairs microscale diffusion properties. Front. Bioeng. Biotechnol. 11:1193970. doi: 10.3389/fbioe.2023.1193970
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Comments
Copyright © 2023 Dogan, Holshue, Bhusal, Shukla and Miri. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).