Date Approved

4-26-2018

Embargo Period

4-30-2018

Document Type

Thesis

Degree Name

MS Pharmaceutical Sciences

Department

Chemistry and Biochemistry

College

College of Science & Mathematics

Advisor

Mugweru, Amos

Committee Member 1

Jonnalagadda, Subash

Committee Member 2

Vaden, Timothy

Keywords

Albendazole, Artemisinin, Cyclic voltammetry, Electrochemistry

Subject(s)

Electrochemistry; Drugs--Analysis

Disciplines

Medicinal and Pharmaceutical Chemistry | Pharmacy and Pharmaceutical Sciences

Abstract

This work is aimed to investigate the metabolic behavior of albendazole and artemisinin. The electrochemical oxidation and reduction of these drugs were performed on electrode materials to mimic their metabolism in vivo using cyclic voltammetry and bulk electrolysis analysis. The oxidation of albendazole on gold electrode surface yielded albendazole sulfoxide and albendazole sulfone which are the main metabolites of this drug in vivo. Reduction of artemisinin on glassy carbon (GC) electrode surface yielded dihydroartemisinin and deoxy artemisinin. The formation of these products was monitored using liquid chromatography and mass spectrometry techniques. The redox processes for both drugs were shown to be irreversible and diffusion controlled. Bioactivation of artemisinin by hemoglobin was detected using a GC/carbon nanofibers (CNFs)/Hb biosensor. The catalytic effect of hemoglobin lowered the reduction potential of artemisinin by 475 mV. The apparent Michaelis-Menten constant (Km) was 0.093 mM which shows excellent biological activity of the CNFs bound hemoglobin. Additionally, the biosensor showed a linear relationship with increasing artemisinin concentration. Therefore, it can be useful to determine concentration of this drug in solution and matrices such as biological fluids.

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