Date Approved

9-20-2024

Embargo Period

9-20-2025

Document Type

Thesis

Degree Name

Master of Science (M.S.)

Department

Chemical Engineering

College

Henry M. Rowan College of Engineering

Advisor

Lark Perez, Ph.D.

Committee Member 1

Gary Thompson, Ph.D.

Committee Member 2

Kenneth Lau, Ph.D.

Committee Member 3

Zhiming Zhang, Ph.D.

Keywords

Electroporation;Food decontamination;Microbial inactivation;Pulsed electric fields;Stress responses

Subject(s)

Farm produce; Microbial contamination

Disciplines

Chemical Engineering | Engineering

Abstract

Several issues exist within the agricultural industry surrounding failure to prevent pathogen transfer to plants preharvest and failure to decontaminate foods postharvest. Fresh produce, like leafy greens, pose a large threat to public safety if contaminated because they are minimally processed and often eaten raw. Numerous outbreaks have been linked to fresh produce in recent decades, with irrigation water emerging as a potential source. These outbreaks have severe economic repercussions and can contribute to millions of hospitalizations and deaths each year, worldwide. This thesis focuses on the usage of pulsed electric fields (PEF) and hydrogen peroxide (H2O2) as a means to create safer food products. First, a threshold is established for when spinach quality deteriorates from monopolar PEF exposures. This provides insight for parameter selection in future studies using bipolar pulses for direct tissue applications. Second, a 5 log reduction of Escherichia coli, Listeria innocua, and Salmonella enterica is achieved through novel split-dose PEF/ H2O2 combination exposures. Third, growth effects of Arugula are investigated in soil by watering using hydrogen peroxide solutions. Decontamination of irrigation water using PEF/ H2O2 treatments may serve as a 2-in-1 solution, where inactivating microbes before watering can prevent whole-field contamination, and residual H2O2 present can enter the soil and prevent fungal diseases like root rot.

Available for download on Saturday, September 20, 2025

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