Date Approved
5-12-2025
Embargo Period
5-12-2025
Document Type
Thesis
Degree Name
M.S. Pharmaceutical Sciences
Department
Chemistry and Biochemistry
College
College of Science & Mathematics
Advisor
James Grinias, Ph.D.
Committee Member 1
Amos Mugweru, Ph.D.
Committee Member 2
Gregory Caputo, Ph.D.
Keywords
Compact Capillary Chromatography;Drugs of Abuse;HPLC;Sample Preparation;SCX;SPE
Abstract
The accurate detection and quantification of drugs of abuse and their metabolites for HPLC analysis is key in the medical, forensic, and environmental sectors. Additionally, the detection of compounds related to substance use disorders in clinical settings can play a crucial role in effective treatment decisions by healthcare practitioners. The ability to detect these compounds at trace levels in any given sample matrix is a known challenge. Coupling a reliable sample preparation method to HPLC analysis for matrix interference reduction and the preconcentration of species for enhanced detection limits may provide a preferred solution to the analysis of drugs of abuse. This study is focused on developing a solid phase extraction method utilizing a polymeric strong cation exchange sorbent coupled with a LC-UV method for the analysis of a 16-compound drugs of abuse panel. Ten-fold recoveries for the 16 compounds ranged from 82.8%-116.9%, with 14 of the 16 compounds falling within the standard recovery range of 90%-110%. This sample preparation strategy and HPLC method was then applied to samples analyzed using a compact capillary LC instrument, with a focus on achieving detection limits below the standard clinical screening thresholds.
Recommended Citation
Kurre, Sangeeta, "METHOD DEVELOPMENT FOR THE ANALYSIS OF DRUGS OF ABUSE USING SOLID PHASE EXTRACTION AND HIGH PRESSURE LIQUID CHROMATOGRAPHY" (2025). Theses and Dissertations. 3352.
https://rdw.rowan.edu/etd/3352
