Date Approved
5-2015
Document Type
Thesis
Degree Name
Master of Science in Biomedical Sciences
Department
Cell Biology
College
Graduate School of Biomedical Sciences
First Advisor
Venkataswar Venkataraman, PhD
Committee Member 1
Robert Nagele, PhD
Committee Member 2
Bradford Fischer, PhD
Subject(s)
Autoantibodies; Blood-Brain Barrier; Calcium-Binding Proteins; Neurodegenerative Diseases; Age Factors; Models, Animal
Disciplines
Animal Experimentation and Research | Cell Biology | Disease Modeling | Genetics and Genomics | Laboratory and Basic Science Research | Molecular Biology | Nervous System Diseases | Neuroscience and Neurobiology
Abstract
Autoantibodies play an important role in many autoimmune diseases. Recent research has shown that breakdown of the blood brain barrier (BBB) occurs concomitant to generation of brain reactive autoantibodies in many neurodegenerative diseases, which serve as biomarkers and drivers of pathology. SI00B, a calcium binding protein found most highly expressed in astrocytes which ensheathe the BBB, has many functions in neural development and signaling. Currently literature indicates that S100B KO mice develop normally, with no phenotypic abnormalities. Here, it is demonstrated that S100B KO mice seem to develop a chronic BBB breakdown similar to that seen in human neurodegenerative diseases. The pattern of breakdown remains similar across multiple brain regions and seems to worsen in a predictable way according to mouse age. This chronic deficiency seems to be associated with changes in the brain reactive autoantibody profile of these mice. Current models of chronic BBB breakdown and subsequent autoantibody generation are lacking; Sl00B KO mice show potential for use as a model for this phenomenon.
Recommended Citation
Brown, Eric V., "Age-Dependent Breakdown of the Blood Brain Barrier and Associated Changes in S100B KO Mice" (2015). Graduate School of Biomedical Sciences Theses and Dissertations. 75.
https://rdw.rowan.edu/gsbs_etd/75
Included in
Animal Experimentation and Research Commons, Cell Biology Commons, Disease Modeling Commons, Genetics and Genomics Commons, Laboratory and Basic Science Research Commons, Molecular Biology Commons, Nervous System Diseases Commons, Neuroscience and Neurobiology Commons
