Document Type

Article

Version Deposited

Published Version

Publication Date

11-22-2023

Publication Title

Scientific Reports

DOI

10.1038/s41598-023-46571-5

Abstract

Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of sample as well as addition of assay-specific reagents and importantly, is a terminal analysis. This study exploits the unique chemical features of a fluorescent molecular rotor that fluctuates between twisted-to-untwisted states, with a subsequent intensity increase in fluorescence depending on environmental conditions (e.g., viscosity). Here we report the development of a rapid, sensitive in situ protein quantitation method using ARCAM-1, a representative fluorescent molecular rotor that can be employed in both non-terminal and terminal assays.

Comments

Copyright 2023 The Authors. Published by SpringerNature. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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