Document Type
Article
Version Deposited
Published Version
Publication Date
11-22-2023
Publication Title
Scientific Reports
DOI
10.1038/s41598-023-46571-5
Abstract
Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of sample as well as addition of assay-specific reagents and importantly, is a terminal analysis. This study exploits the unique chemical features of a fluorescent molecular rotor that fluctuates between twisted-to-untwisted states, with a subsequent intensity increase in fluorescence depending on environmental conditions (e.g., viscosity). Here we report the development of a rapid, sensitive in situ protein quantitation method using ARCAM-1, a representative fluorescent molecular rotor that can be employed in both non-terminal and terminal assays.
Recommended Citation
Daus, K., Tharamak, S., Pluempanupat, W. et al. Fluorescent molecular rotors as versatile in situ sensors for protein quantitation. Sci Rep 13, 20529 (2023). https://doi.org/10.1038/s41598-023-46571-5
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Comments
Copyright 2023 The Authors. Published by SpringerNature. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License