Date Approved

4-20-2023

Embargo Period

4-26-2023

Document Type

Thesis

Degree Name

M.A. Pharmaceutical Sciences

Department

Chemistry and Biochemistry

College

College of Science & Mathematics

Advisor

Lark Perez, Ph.D.

Committee Member 1

Subash Jonnalagadda, Ph.D.

Committee Member 2

Kandalam Ramanujachary, Ph.D.

Keywords

bioluminescent bacteria

Subject(s)

Organic photochemistry

Disciplines

Chemistry | Organic Chemistry

Abstract

Bacteria, though well-known and widespread in scientific application, have plenty more opportunity to grow in the field of organic synthesis. The primary objective of this project was to apply the use of bioluminescent bacteria in an organic photoredox reaction intending to use the bacteria as a renewable source of light. This sustainable method, as opposed to high wattage bulbs, paves a green pathway for organic photocatalytic reactions. Using bioluminescent E. coli, the focus was on performing organic oxidation reactions with a recyclable photocatalyst. When using bacteria in conjunction with chemicals like nitromethane, it was an obstacle to keep the E. coli alive. By employing toxicology studies, we could determine minimum inhibitory concentration (MIC) values for each chemical that were to be tested. Setting up control experiments and reproducibility studies was necessary in order to confirm the possibility of such reactions. With these constraints and variables under control, we were able to perform our photoredox reactions successfully using bioluminescent E. coli as our light source and a graphitic carbon nitride polymer as our photocatalyst. The reactions targeted in this study gave way to a high yield product, confirming that bioluminescent bacteria can be used as a light source in solution with organic solvents.

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